| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061363 |
| Chromosome: | chromosome 12 |
| Location: | 4048764 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516350 | COX10 | Mitochondrial cytochrome c oxidase assembly protein; (1 of 1) K02257 - protoheme IX farnesyltransferase (COX10) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTTTTCGGGCTGCGACAACTGCTATGCTAAAATTTTGTTGCATGGGC |
| Internal bar code: | GTGATCGCAGGAGTGGGCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1278 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTTCTCCATCCACTGTT |
| Suggested primer 2: | AATCCCCTTGGTGCTGGATG |