| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061382 |
| Chromosome: | chromosome 13 |
| Location: | 3796053 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACCTTCAGCAGTGCGTAGGCTTACAATTCAATAGTAAGGTAAACTACC |
| Internal bar code: | TACACGGACGTACATCTATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3752 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGCCAAGAAGACGAGTG |
| Suggested primer 2: | GGTTTGGGTTTGCGACTGTC |