| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061383 |
| Chromosome: | chromosome 6 |
| Location: | 7064333 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297650 | UBC36 | (1 of 1) K10582 - ubiquitin-conjugating enzyme E2 Q (UBE2Q); E2 ubiquitin conjugating enzyme | 3'UTR |
| Cre06.g297700 | (1 of 1) IPR013026//IPR019734//IPR027417 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCAAGTCAGGAGCAGCCCAAACACTGTCATAGCTTACACGTCAGTGT |
| Internal bar code: | TGTTCAGTTATGCGTCTTTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4765 |
| LEAP-Seq percent confirming: | 98.2456 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGACCTGTTTCGGAAGG |
| Suggested primer 2: | TGTTTGACAACATGCAGGCG |