| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061391 |
| Chromosome: | chromosome 10 |
| Location: | 1698810 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429750 | FAP417 | (1 of 1) IPR000014//IPR000104 - PAS domain // Antifreeze protein, type I; Flagellar Associated Protein 417 | 3'UTR |
| Cre10.g429800 | COQ8,ABC1 | Ubiquinone biosynthesis protein; (1 of 1) PTHR10566:SF65 - UBIQUINONE BIOSYNTHESIS PROTEIN COQ-8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGGAAAGGCGGCTAGCAGCTGTGTTTGCGGCCGGGCTGGAGTGTCG |
| Internal bar code: | TATAGGGAGTATTGTAAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2612 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTTACAGTACCCGCGTG |
| Suggested primer 2: | ACGAGCCAGTGGTCCTATCT |