| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061397 |
| Chromosome: | chromosome 3 |
| Location: | 8783229 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207265 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTAGTACATAATAGCATGCCATGCGTGCACCCACTTCGAATCCGTCG |
| Internal bar code: | TGTCAATTAGCGATTGCGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2044 |
| LEAP-Seq percent confirming: | 94.3662 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTTAAGGTTTGCACGCA |
| Suggested primer 2: | GTCGGTGGGTTTAGACAGCA |