| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061403 |
| Chromosome: | chromosome 12 |
| Location: | 6270949 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCAACCCCGACTTTGCTGCAAACCCTCCCGCGGGCAAAGTCCGTGTG |
| Internal bar code: | AATGTTAACGGATGCCTTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4642 |
| LEAP-Seq percent confirming: | 53.6082 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTTGGGACTGCACTGT |
| Suggested primer 2: | CGGTCTGCCCTTTGAGCTTA |