| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061417 |
| Chromosome: | chromosome 12 |
| Location: | 5684285 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532000 | (1 of 3) PF04359 - Protein of unknown function (DUF493) (DUF493) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTGGCCTTGAGCGGCCTTGCAACGTGACGGTGTGGCCGTGTGGGTC |
| Internal bar code: | TTACTAGATTGATTTAAGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2639 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGCGCCATCAAGGAATG |
| Suggested primer 2: | GGCTGTGTCAAACGTGCAAT |