Insertion junction: CLIP2.061418_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.061418
Chromosome:chromosome 13
Location:2064844
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g576900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGGAGAACGCCGTGAAGAAGAGTTGTGGCTGGGGGAACATCCATTGT
Internal bar code:GCAACCCAGTCACATACCGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2073
LEAP-Seq percent confirming:96.2963
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTTCCCGGATCCCAACC
Suggested primer 2:AGGGCACACACTGTCTAAGC