| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061425 |
| Chromosome: | chromosome 3 |
| Location: | 2109542 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156550 | (1 of 1) PTHR22829//PTHR34826 - DEP DOMAIN PROTEIN // FAMILY NOT NAMED | 5'UTR | |
| Cre03.g156600 | GluTRBP,GTRBP1 | (1 of 1) PTHR13343//PTHR13343:SF14 - CREG1 PROTEIN // GENOMIC DNA, CHROMOSOME 3, P1 CLONE: MXL8; Glutamyl-tRNA reductase binding protein 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGGCCGGGTGCAGCAAAAGGAAGCCACAGCGACTTCTATTACAGGGT |
| Internal bar code: | GAGTATGCGGGACATTAGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2784 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTAACGCTGCAAAACC |
| Suggested primer 2: | ATCAGGCTGCAGGAGTTCAC |