| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061431 |
| Chromosome: | chromosome 12 |
| Location: | 2265337 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508550 | PANB,PAN2,PANB1 | (1 of 1) 2.1.2.11 - 3-methyl-2-oxobutanoate hydroxymethyltransferase / Ketopantoate hydroxymethyltransferase; Ketopantoate hydroxymethyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTTTCCCTTAACTTTGCCTGGAACTCGTTGCTCAGCTTTACTTTCAG |
| Internal bar code: | GTTGAGGTCTGTTATTGTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2589 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGGGAGTGTCGAGCTTG |
| Suggested primer 2: | CACAGTGTTCCTGGTACCCC |