Insertion junction: CLIP2.061433_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.061433
Chromosome:chromosome 1
Location:7992563
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g055966 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGCCACCGCCACCGCCACCACCACGGCCACCACCGCCGTCTGCCGGC
Internal bar code:TTATTGGAGAAAACCACGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:206
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCACAACCACAACCAGT
Suggested primer 2:CACCACATCCACCACATCCA