Insertion junction: CLIP2.061435_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.061435
Chromosome:chromosome 12
Location:5269859
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g527918 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACGGATTACTCGTGCGCAACGTGTAGCCGTCGGCATGGAACAGAGGC
Internal bar code:ACACAACTCGGTACTCCATTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3739
LEAP-Seq percent confirming:97.3684
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GATGACCCAGGAAGTGGTGG
Suggested primer 2:CACTACGACCGGTGGAACAA