| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061473 |
| Chromosome: | chromosome 13 |
| Location: | 3888723 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589700 | VPS20 | Subunit of ESCRT-III complex; (1 of 1) K12195 - charged multivesicular body protein 6 (CHMP6, VPS20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTGCTTGCACACTTGAATTTTCCCTTTTGTAACGTCGCAAGATAAC |
| Internal bar code: | TATAGGTTGGGGCTCCATGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1128 |
| LEAP-Seq percent confirming: | 52.7473 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTGGTGGTGGATGAAG |
| Suggested primer 2: | CAGAGGCGTTAGTTGCAAGC |