Insertion junction: CLIP2.061477_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.061477
Chromosome:chromosome 16
Location:7755481
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g687182 POB10 Proteome of basal body 10; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCGAGAGAAGTCCGTAGACCTCTTGTTACACCGAGGCGGGGGCGAAC
Internal bar code:TACGCCCGGTAGGGGTCGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1775
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGCATCGCCAAAGTATG
Suggested primer 2:GCTGTGCCAGGAACAAGTTG