| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061482 |
| Chromosome: | chromosome 17 |
| Location: | 1435019 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g706400 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACGACTGTGCATGTGACACGGTTACTCGGTACGGCCGCACGCCGGCC |
| Internal bar code: | CTACTAGTGGTTTATCGTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2009 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCACCGGTGTGAAGTTG |
| Suggested primer 2: | AGTGGGTGTTTTGACTGGCA |