| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061487 |
| Chromosome: | chromosome 12 |
| Location: | 2547324 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506900 | (1 of 1) PTHR21568:SF0 - TRNA PSEUDOURIDINE SYNTHASE PUS10-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATCAGCTCACCCCCAGCCCCCTGCACAGCTACCCTGCTCCCAGTCGCG |
| Internal bar code: | AAATTTGGTGAGTTTTGTTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGCAAGCCTGTAAGCGA |
| Suggested primer 2: | CACGGGTAGGGATATCGCAC |