| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061525 |
| Chromosome: | chromosome 7 |
| Location: | 6439625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357950 | (1 of 1) PF00646//PF12796 - F-box domain (F-box) // Ankyrin repeats (3 copies) (Ank_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATAAGCGGACGCGCTGGGCGCGTTAGGATGCGTGGGGTTCAGCGCGG |
| Internal bar code: | CGAGATTGAGCAAGATGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCAACACACCTGATACT |
| Suggested primer 2: | TGTGCTGCCTCAGAGACTTG |