| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061531 |
| Chromosome: | chromosome 16 |
| Location: | 7797514 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688078 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTGTCCACGGAATGGTGCGCTCCATGGTGGGGCACTTTCATGTGCAT |
| Internal bar code: | TTTGCAGGTAGGAGCATGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2204 |
| LEAP-Seq percent confirming: | 89.1026 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 156 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTAGCGTACGTAGCGTCG |
| Suggested primer 2: | TACGACACAGAGACCGACAC |