| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061545 |
| Chromosome: | chromosome 1 |
| Location: | 7717614 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054300 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCGCTGGAACAGCGCCCCGTCCAAGAACCCCGTGAAGAATCCAGCAT |
| Internal bar code: | AACAGTTCTTTTGTCATGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1595 |
| LEAP-Seq percent confirming: | 86.3014 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 146 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGCGACTTGTGACGTTA |
| Suggested primer 2: | GATAGTTCCAAGCTTGCGCG |