| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.061546 |
| Chromosome: | chromosome 9 |
| Location: | 5370409 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408000 | POB22 | (1 of 3) PF03227 - Gamma interferon inducible lysosomal thiol reductase (GILT) (GILT); Proteome of basal body 22 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCTCATCTGCATCCAATATGCATGCGCGCATGCGTGTGCACGTGCAC |
| Internal bar code: | GGTATGACCCGTGTACGGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 826 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGCAACTTGGGTCTTG |
| Suggested primer 2: | CGCGTAGAAATTCTGCTGGC |