Insertion junction: CLIP2.061555_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061555
Chromosome:chromosome 3
Location:1117975
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g148800 (1 of 1) 2.1.1.4 - Acetylserotonin O-methyltransferase / Hydroxyindole O-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTTGTCTAAGGATTCCCGCACGCGCCGAGGTTTGCCTGTCCGATAGAC
Internal bar code:ATTCGTGATTCCGGCTGCCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4146
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCCACGAAACTTCAACG
Suggested primer 2:GTGACAAGGAAACGGTTGGC