Insertion junction: CLIP2.061567_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.061567
Chromosome:chromosome 2
Location:791083
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTTTTAGTTTCCCCGCAATGACGAATGCCGCGTTCTGTATTCTGCTCG
Internal bar code:GTTCCATGAATCGCTGGGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1912
LEAP-Seq percent confirming:76.7442
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CATAGCCATTGACGCAGCAC
Suggested primer 2:GCGCACATGTTTCGGTATCC