| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061569 |
| Chromosome: | chromosome 10 |
| Location: | 5934092 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461000 | (1 of 1) K14772 - U3 small nucleolar RNA-associated protein 20 (UTP20) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGTTCGGCCAGTTGTGTTTTGACGTCTGCAACCCTCGCTCACCCTCT |
| Internal bar code: | ATCTGTCATTGCGAACTCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 7.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTGAGGGTTGCAGGCAT |
| Suggested primer 2: | GCTGAAGAAGGTCCCCAACA |