Insertion junction: CLIP2.061576_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.061576
Chromosome:chromosome 1
Location:5517563
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g039250 RPS2 Cytosolic 80S ribosomal protein S2; (1 of 1) K02981 - small subunit ribosomal protein S2e (RP-S2e, RPS2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACAAGGAGGTGGCTGCCTTAGGGAGGCAGGAAAAGGTCTGCTGCGTCT
Internal bar code:TATACCCCTAATGTTGTCATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4032
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:CGATACCCAAGGCGGCTTAT
Suggested primer 2:TGCTCAGGACAACGATGCAT