Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.061577 |
Chromosome: | chromosome 1 |
Location: | 7609366 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g053300 | AIH1 | Agmatine iminohydrolase; (1 of 2) 3.5.3.12 - Agmatine deiminase | outside_mRNA |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGAAGGGGTGCACGTGTGCAGTCGCAATACGGCAGTGCCTGTCCTGA |
Internal bar code: | GTAAAAGGACTTGGTAAGTGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1444 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 28 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTTGAGTTGCTTGCGTTGA |
Suggested primer 2: | TCTGATCAGACGAGTCGGGT |