| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.061585 |
| Chromosome: | chromosome 3 |
| Location: | 8089669 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201100 | FTSHi1,FTSHI4,FTSHi4,FHL1,FHL6 | (1 of 1) PTHR23076//PTHR23076:SF61 - METALLOPROTEASE M41 FTSH // SUBFAMILY NOT NAMED; Chloroplast-import FtsH-like ATPase | 5'UTR |
| Cre03.g201150 | (1 of 2) 2.5.1.117 - Homogentisate solanesyltransferase / HST | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAAACTGTCAATTTGCAGGATGGTTTTCACCTCGCAATGAAGCGTTA |
| Internal bar code: | CATTAGTGCCCTATGCAGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGCGAGTTGAGTTGCA |
| Suggested primer 2: | GAAGAGTCGAACAAGGGGCA |