| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.061592 |
| Chromosome: | chromosome 5 |
| Location: | 3427764 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233304 | CHI3 | (1 of 2) K01183 - chitinase (E3.2.1.14); Chitinase-like hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACATGGCACTTAGTTGTTATGAAGCAGCTTCGGCACGTGAGGGGTGG |
| Internal bar code: | ATTAACCTCGTTGGTTCGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1388 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGCCCTCCCTAGTTGAT |
| Suggested primer 2: | TGCAGCCCCTTGTAGTCTTG |