| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061602 |
| Chromosome: | chromosome 8 |
| Location: | 1481829 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364751 | BLZ16 | bZIP transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGCACGTTGAGACTGCATACCACTCCAAGGTACGGCTGAGACGCAAC |
| Internal bar code: | ATACGAAAAGACCATAACATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1390 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCCTGACTGGTCCCTAC |
| Suggested primer 2: | GTTTGAGCCCCAACAGCTTG |