| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061603 |
| Chromosome: | chromosome 17 |
| Location: | 1811004 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709050 | HTR3,HTR28 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAACACCGAGTTTTTTGGTTGCGCCTCCCGCTACCGTCACGCCCGAAA |
| Internal bar code: | AGGAAGTGGGGATTGACAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTGACCAGACGTTGAAC |
| Suggested primer 2: | CCGTGACCTGCACTACTGTT |