| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061608 |
| Chromosome: | chromosome 1 |
| Location: | 8063516 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g062172 | HBV1 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B variant | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTGACACTATAACAAAATGGCTCCCAAGGCTGCTGAGAAGGCTCCGG |
| Internal bar code: | ACGCGGTTAAAGTACTATCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3740 |
| LEAP-Seq percent confirming: | 98.9583 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTGGCCATCTTGTCGAA |
| Suggested primer 2: | ATGGCAGTTTGTGTTTCGCC |