| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061615 |
| Chromosome: | chromosome 14 |
| Location: | 3415788 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801654 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTAAGGATTTCGACATTCCCGACCCGAAAGGACCATTCTTTGATTCA |
| Internal bar code: | AGTGGTATTAGAAGACATGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4316 |
| LEAP-Seq percent confirming: | 50.9091 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTGTCAAGATGCAGGCT |
| Suggested primer 2: | TGCGACGCAAACTTTGTGAG |