| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.061623 |
| Chromosome: | chromosome 6 |
| Location: | 8212763 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306300 | CHLI1 | Magnesium chelatase subunit I, isoform 1; (1 of 2) PTHR32039:SF9 - MAGNESIUM-CHELATASE SUBUNIT CHLI-2, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCTGATAACCCTCTAGCTCATGCCTCTAGCTTCAGGTGAGACCTCGC |
| Internal bar code: | ATTGGGCCGGCACGTGTTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3400 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGGCCTATTCTGCAATG |
| Suggested primer 2: | AGGCCATGTTAATGCCTGCT |