| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.061630 |
| Chromosome: | chromosome 17 |
| Location: | 2541800 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715900 | Similar to Dihydrofolate Reductase-Thymidylate; (1 of 1) 1.5.1.3 - Dihydrofolate reductase / Tetrahydrofolate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCTATGGCTTAATGGATAAAGCGCCGGTCTACGGAGAATAAGCTGTG |
| Internal bar code: | GAGGTGTCTCGTCGTAGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1293 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAGGATCGTTTTGGCCC |
| Suggested primer 2: | CAGCCATGTCCATTCATGCG |