| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.061635 |
| Chromosome: | chromosome 16 |
| Location: | 2401020 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659650 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGATCAGTTAAGAAGTGAAGGCGTAGCCAGGGGGAGGGGGATGCGGTG |
| Internal bar code: | AAGGACGCGTGGATTTGTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 27.5862 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTAGAGGGACCCGAGAT |
| Suggested primer 2: | GCGTATGCGATGCAATGTGT |