| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.061649 |
| Chromosome: | chromosome 5 |
| Location: | 1515445 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234658 | (1 of 1) PTHR13480:SF0 - E3 UBIQUITIN-PROTEIN LIGASE HAKAI | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGTCGAGGCGTGAAGGCTTGAGCAGTGGCAGCAGGTGGCTTGCGTTG |
| Internal bar code: | GCGGTGTGGCGGGGGGTGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1167 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGATGTTGGAATGCTGG |
| Suggested primer 2: | TGTGATGTCGGTGATGGGTG |