| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061658 |
| Chromosome: | chromosome 7 |
| Location: | 2968690 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g333000 | CEP4 | (1 of 1) 3.4.22.15 - Cathepsin L; Cysteine endopeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTGTTATTGCCTATTGCATGTAAATAGGCGGCGGGGAAAATTCGGGC |
| Internal bar code: | CAGTGCGTCGACAAATACCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3709 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAACCAACGAGCCACAC |
| Suggested primer 2: | CGGACGGGGAGATGAAGATG |