Insertion junction: CLIP2.061660_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061660
Chromosome:chromosome 16
Location:4627313
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670750 (1 of 1) IPR012312//IPR012827 - Haemerythrin-like // Haemerythrin, metal-binding domain 3'UTR
Cre16.g670754 (1 of 1) PTHR10217//PTHR10217:SF512 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCAAACCCGGGCCACCAAAGTCTCGCCCCAGACATTAGCACCCAGGA
Internal bar code:GTACGAAAGTGGAGACAAGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:609
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATTACTGACCGTGCTTGGGG
Suggested primer 2:AGCAGAAACAGAGACGGGTG