Insertion junction: CLIP2.061698_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.061698
Chromosome:chromosome 9
Location:2999024
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393506 HCP3 (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGTACGAATCCCGCTCCGGGACGCTCCTAGGGGCAGTCACCTGTGCGC
Internal bar code:TATTAGGCTTTTCAGCAGGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:5118
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAGCGCCTCAATGATTGG
Suggested primer 2:CCGAAAGCAAAGAACGCACA