Insertion junction: CLIP2.061702_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.061702
Chromosome:chromosome 1
Location:2904902
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g018075 3'UTR
Cre01.g018100 (1 of 1) 3.1.3.16//3.1.3.27//3.1.3.48 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Phosphatidylglycerophosphatase // Protein-tyrosine-phosphatase / PTPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCTTCTCATGCGTTCTCACCACCATCTGCTCTTGCTCGTACTGGGAA
Internal bar code:TGTTGGGATGTTCGCGGTCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:3716
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:97

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGTCACAGTCCGCTTGTC
Suggested primer 2:ACATCAGCAGCTGAACACGA