| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061703 |
| Chromosome: | chromosome 12 |
| Location: | 5874466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGGTCCCAGTGGGATGATGGGTACAGGAATGGCCCAGGATGCGCCAG |
| Internal bar code: | GCTACCTACTTTGCCGTTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4854 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGAACCCTGTCCCATCAC |
| Suggested primer 2: | AGCCCAGTCCTCCTCATCAT |