| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.061704 |
| Chromosome: | chromosome 16 |
| Location: | 947750 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648600 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCACCATGGGTTGCCAAGCCACCTGCCTAATATGTCACCAGCCCTGT |
| Internal bar code: | ACTATGGACGTCGTTAAAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2731 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCAGTGAGGATGTGCTC |
| Suggested primer 2: | CGTATAGCAGTACAGCGCCA |