| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.061722 |
| Chromosome: | chromosome 2 |
| Location: | 5315950 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTAGATCGCTGTCAATACTGCAGCATGACAGCACGCGCCATCACAAGT |
| Internal bar code: | TTTTCTCCAGTACGATGTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5714 |
| LEAP-Seq percent confirming: | 97.2477 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCGTTGTTACCACAGCT |
| Suggested primer 2: | GGCTGAACGTCACAAGAGGA |