Insertion junction: CLIP2.061725_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.061725
Chromosome:chromosome 7
Location:1551781
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g324800 SNE6 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGAGGCGTGTGCAATTTGCGTGTGCTTAGTAGGCGCCCGCGTCAAGGTG
Internal bar code:GAATTTGAAGGAAGAGCTGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2473
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:96
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:96

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATTGCTGCGTCGGTTTG
Suggested primer 2:GTGACTCTTGTCCCTGGCTC