Insertion junction: CLIP2.061725_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.061725
Chromosome:chromosome 7
Location:1551781
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g324800 SNE6 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTGGGGGCCTTTGGCATCCCCGCAAATCCGGACGTGGCACATGAGATG
Internal bar code:GAATTTGAAGGAAGAGCTGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:958
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACTCTTGTCCCTGGCTC
Suggested primer 2:TTGATTGCTGCGTCGGTTTG