| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061740 |
| Chromosome: | chromosome 3 |
| Location: | 6557257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194400 | EIF3I | Eukaryotic translation initiation factor 3, subunit I; (1 of 1) K03246 - translation initiation factor 3 subunit I (EIF3I) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCTTATGCACGTGTCTACGCCTGGCGCTTGGAGCCCATTAATGGTTC |
| Internal bar code: | CAATTAAACATTTACTGATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3259 |
| LEAP-Seq percent confirming: | 88.2759 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 145 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGTAACATCGCGGCTCG |
| Suggested primer 2: | CAATGGTGGGTGGTAGGACC |