Insertion junction: CLIP2.061748_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.061748
Chromosome:chromosome 3
Location:750039
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g146027 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGGGCATTCAGTGCTCAGGCGGTGGCGGTAGCAGTTAAGTGTGGCTGG
Internal bar code:GTTATAGAGTTTGGTGAGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1528
LEAP-Seq percent confirming:1.3245
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:149
LEAP-Seq n unique pos:151

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGTGTGTACAGTCTGGA
Suggested primer 2:CCCCTGACGCACATGTGTAT