| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061774 |
| Chromosome: | chromosome 1 |
| Location: | 8205267 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g071662 | ACS1 | Acetyl-CoA synthetase/ligase; (1 of 3) K01895 - acetyl-CoA synthetase (ACSS, acs) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCATGTGTCCATGGCCGACCACCGACCCTTTTGTCAGACTACCGGG |
| Internal bar code: | AAGGTCTCGATCACATCATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3493 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAAGTTCAGGGTCGGTCG |
| Suggested primer 2: | AGGTCACCAAGCATCACCTG |