Insertion junction: CLIP2.061792_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.061792
Chromosome:chromosome 1
Location:3358915
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g021800 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCACTCTCCTTGCAAATTGCAGTGCAGCCCACCGCACTTGTGGGCACT
Internal bar code:ACTTTATCGATTATTTCGCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:469
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTAGTGCCGACCCAACA
Suggested primer 2:CAACAACCACCCTTGAAGCG