Insertion junction: CLIP2.061811_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.061811
Chromosome:chromosome 6
Location:1675167
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g262300 CYN51 Cyclophilin 51; (1 of 2) K05864 - peptidyl-prolyl isomerase D [EC:5.2.1.8] (PPID, CYPD) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTACCCCACACTGTGACCCGGGCACCTCTGAGAGAGCCAACTCATCGC
Internal bar code:CCCACAACTTGGTTAGGCTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1202
LEAP-Seq percent confirming:96.2963
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTTTGTGTGTACCGGC
Suggested primer 2:GCCCGTGTAGATCCACAACA