Insertion junction: CLIP2.061825_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.061825
Chromosome:chromosome 6
Location:7735912
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g302800 STK (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK); Serine/threonine protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAGCTATGCGCCTTGCCAAAGTGCTGCCTGTCCTCCTTGCCGCACCTC
Internal bar code:ATTGGTTCTTAATCAAGTGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4860
LEAP-Seq percent confirming:99.2806
LEAP-Seq n confirming:138
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:139

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAATCTGTCAACGATGGC
Suggested primer 2:AACAGCGCTTGCCTTCAAAG